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Fig. 6 | Molecular Horticulture

Fig. 6

From: Histone deacetylases repress the accumulation of licochalcone A by inhibiting the expression of flavonoid biosynthetic pathway-related genes in licorice (Glycyrrhiza inflata)

Fig. 6

Analysis of flavonoids contents in OE-GiHDA2b and RNAi-GiHDA2b transgenic hairy roots. A The phenotype of the OE-GiHDA2b and RNAi-GiHDA2b transgenic hairy roots (scale bars: 1 cm). B Expression level of GiHDA2b in the OE-GiHDA2b and RNAi-GiHDA2b lines as determined by qRT-PCR. C Immunoblot analysis of histone acetylation in WT, OE-GiHDA2b and RNAi-GiHDA2b hairy roots. ImageJ software was utilized to quantify band intensities. Histone acetylation levels were detected using antibodies against H3ac, H3K9ac, H3K14ac, H3K18ac, and H3K27ac, with histone H3 serving as the internal control for nuclear protein loading. The red numbers indicate the relative quantitative values of band intensities. The content of total flavonoids (D), LCA , isoliquiritin (F), echinatin (G), licochalcone C (H) and daidzein (I) in OE-GiHDA2b and RNAi-GiHDA2b transgenic hairy roots were quantified by HPLC. The non-transgenic hairy roots were designated as WT. The different lower-case letters indicate a significant difference at the 0.05 level for the relative expression level and flavonoids content among samples. Student’s t-test, n = 3

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Molecular Horticulture

ISSN: 2730-9401

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