Fig. 5
Expression profiles of GiHDAC genes and histone acetylation status under different treatments. The content of total flavonoids (A), LCA (B), isoliquiritin (C), echinatin (D), licochalcone C (LCC) (E), and daidzein (F) in G. inflata seedlings under various treatments were quantified using HPLC. (G) The morphology of G. inflata seedlings under different treatments. Five-day-old G. inflata seedlings were exposed to ethanol (Control), 250 mM NaCl, NaHCO3(pH 8.0), cold (4℃), 100 μM MeJA and 100 μM ABA for 7 days. Scale bar = 2.0 cm. H The relative intensity was calculated as the ratio of histone H3 acetylation to total histone H3 using ImageJ. I, J qRT-PCR analysis of gene expression. Different lower-case letters indicate statistically significant differences at a P-value of 0.05 for the relative expression level and the flavonoid contents among samples. Student's t-test, n ≥ 3. The y-axis depicts expression levels relative to the control, which was set to 1.0 (indicated with a dashed line). I and J include 8 representative GiHDAC genes from different families