Fig. 4

Characterization of GiHDACs. A Phylogenetic tree construction based on the full-length sequence of GiHDAC proteins. Colors denote subfamilies: red for RPD3/HDA1, blue for HD2, and green for SIR2. B Distribution of motifs in each GiHDAC protein. Motifs and their gene locations are labeled and classified according to subfamilies. C Gene structure and conserved domain distribution of GiHDACs. Green boxes represent unrelated areas, black lines indicate introns, yellow boxes show HDAC domains, orange boxes denote NPL domains, and purple boxes represent SIR2 domains. D Subcellular localization of GiHDACs. GiHDACs-GFP fusion constructs were utilized to determine the subcellular localization of GiHDACs. NLS-mCherry served as a nuclear marker. Scale bars = 10 μm