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Fig. 7 | Molecular Horticulture

Fig. 7

From: AcNAC10, regulated by AcTGA07, enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae via inhibiting jasmonic acid pathway

Fig. 7

AcTGA07 promotes AcNAC10 expression by directly binding to the JA-responsive element (TGACG-motif) of the AcNAC10 promoter. A. Dual luciferase assay confirmed the activating effect of AcTGA07 on the AcNAC10 promoter. B. Quantitative analysis was performed to measure the LUC/REN ratio. ①, 62SK-AGTGA07 + 0800-proAcNAC10. ②, 62SK + 0800-proAcNAC10. C. GUS activity was used to analyze the activation effect of AcTGA07 on the AcNAC10 promoter region. The statistical significance of the results was determined using Student's t-test, where "ns" indicates not significant, *, p < 0.05; **, p < 0.01 and ***, p < 0.001. Values represent mean ± SD of three technical replicates. D. Yeast one-hybrid (Y1H) assay confirmed the ability of AcTGA07 to bind to the TGACG-motif. E. EMSA demonstrated that AcTGA07 binds directly to the TGACG motif in the AcNAC10 promoter

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Molecular Horticulture

ISSN: 2730-9401

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