Fig. 5

AcNAC10 binds to the AcLOX3 promoter and suppresses AcLOX3 expression. A and B Luciferase assays in tobacco leaves. p62SK-EV, p62SK-empty vecto; p0800-EV, pGreenII0800-empty vectors. Student’s t-test determined significant differences between relative expression levels at ** p < 0.01. C The ratio of dual luciferase reporter LUC/REN. D Schematic diagram of the yeast one-hybrid vector construction using pHis2 and pGADT7. AD and pHis-p53 (positive control) in Y187 cells. The AD-AcNAC10 (pGADT7-AcNAC10) and pHi2-proAcLOX3 combination in Y187 cells was used as the experimental group. The pGADT7 and pHis2-proAcLOX3 were used as the controls. E Schematic diagram of the AcLOX3 promoter structure and Yeast one-hybrid assay validating that AcNAC10 can interact with the NACRS site of the AcLOX3 promoter. F. Electrophoretic mobility shift assay (EMSA) validated that AcNAC10 can bind to the NACRS site