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Fig. 8 | Molecular Horticulture

Fig. 8

From: RsWRKY40 coordinates the cold stress response by integrating RsSPS1-mediated sucrose accumulation and the CBF-dependent pathway in radish (Raphanus sativus L.)

Fig. 8

RsWRKY40 enhances its own transcription level. A The schematic diagram of the prey and bait constructs used in the Y1H assay. B RsWRKY40 binds to its own promoter. C The schematic diagram of the effector and reporter constructs used in the dual luciferase assay. D, E RsWRKY40 activates its own expression as indicated by LUC fluorescence images (D) and the relative LUC intensity (E). The LUC fluorescence images and relative LUC intensity were obtained by using a chemiluminescence imaging system and a Clinx IVScopeEQ Capture software, respectively. Data are presented as the mean ± SD; n = 3 (***P < 0.001, Student’s t-test)

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Molecular Horticulture

ISSN: 2730-9401

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