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Fig. 5 | Molecular Horticulture

Fig. 5

From: CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus

Fig. 5

Regulatory effects of CitHAG28 on the GS pathway associated gene. A Expression patterns of CitACO3, CitIDH1, and CitGS1 in CitHAG28-OE citrus callus. B Expression patterns of CitACO3, CitIDH1, and CitGS1 in CitHAG28-TRV citrus seedlings. C The schematic map of primer design positions for ChIP-qPCR assay. D The levels of H3ac around the promoter and near the TSS of CitACO3, CitIDH1, and CitGS1 in CitHAG28-OE citrus callus. E The levels of H3ac around the promoter and near the TSS of CitACO3, CitIDH1, and CitGS1 in CitGATA7-OE citrus callus. Error bars represent the SE (n = 3). Asterisks denote significant differences using Student's t-test (*p < 0.05; **p < 0.01; ***p < 0.001). ACO, aconitase; ChIP, chromatin immuno-precipitation; GS, glutamine synthetase; H3ac, Histone 3 acetylation; IDH, isocitrate dehydrogenase; TSS, transcription start site

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Molecular Horticulture

ISSN: 2730-9401

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