Fig. 7
Long non-coding RNA lncRNA809 promotes the expression of MmNAC17 and promotes catechin biosynthesis. A-D LncRNA omics analysis and determination of lncRNA809. A Classification of lncRNAs. LncRNAs are divided into 5 categories according to the position of lncRNA and mRNA. B Proportional of 5 types of lncRNAs in Group 1 and Group 4. C Venn diagram of differentially expressed lncRNAs whose levels were significantly affected by sorbitol (H2O versus sorbitol and H2O + R1-infected leaves versus sorbitol + R1-infected leaves). D Analysis of collinearity between lncRNAs and MmNAC17. The lncRNA labeled with a red background has the highest correlation with MmNAC17. E-G Gene expression in different backgrounds. E Relative expression levels of lncRNA809 and MmNAC17 under sorbitol feeding and pathogen R1 infection. The structure of lncRNA809 is shown in the upper right corner. (F) Relative expression levels of lncRNA809 and MmNAC17 in leaves overexpressing lncRNA809. G Relative expression levels of MmF3H and MmLAR in lncRNA809-OE and lncRNA809-OE/MmNAC17-RNAi leaves. Transcripts were quantified by RT-qPCR. H The content of catechin in MmNAC17-OE and lncRNA809-OE/MmNAC17-RNAi leaves. Catechin were quantified by HPLC. The data is the mean ± SD (n = 3). *P < 0.05, **p < 0.01, ***p < 0.001, two-sided Student’s t-test