Fig. 5
From: Allelic variation in an expansin, MdEXP-A1, contributes to flesh firmness at harvest in apples
Transcriptional activity analysis of the structural variation and identification of a regulatory transcription factor, MdNAC1. A B-glucuronidase (GUS) staining of the truncated fragment of the MdEXP-A1 promoter in transgenic tobacco leaves. B The relative activity of GUS in the truncated promoter of MdEXP-A1. C Transcript accumulation of MdNAC1 in ‘Fuji’, ‘Cripps Pink’, and ‘Ruixue’ fruits during development. D Expression patterns of MdNAC1 in parents of ‘Ruiyang’ and ‘Scilate’ fruits throughout the entire developmental stage. E Subcellular localization signal of the MdNAC1 protein in tobacco leaves. Scale bars = 20 μm. F Transcriptional activation activity analysis of the MdNAC1 protein in the yeast system. The error bars represent the standard error of 3 replicates. Asterisks indicate a significant difference determined via a t test (**P <0.01)