Fig. 3
From: Allelic variation in an expansin, MdEXP-A1, contributes to flesh firmness at harvest in apples
Identification of the MdEXP-A1 gene correlated with flesh firmness. A Phylogenetic relationship analysis of MdEXP-A1 in apples and orthologs in diverse species. B Expression patterns of MdEXP-A1 in fruits of ‘Fuji’, ‘Cripps Pink’, and ‘Ruixue’ fruits during various developmental stages. C Relative expression analysis of MdEXP-A1 in diverse apple tissues via RT‒qPCR. Different letters represent significant differences between diverse tissues. Significance was defined at P < 0.05 (Student’s t-test). D Transcription level of MdEXP-A1 in super-hard (Del-1166) and super-soft (Del/del-1166) F1 hybrids at harvest period. E Expression level of MdEXP-A1 in apple fruits after treatment with four hormones. F Relative expression level of MdEXP-A1 in the parents of ‘Ruiyang’ and ‘Scilate’ at various developmental stages. G Subcellular localization of the MdEXP-A1 protein. Scale bars = 20 μm. FPKM: Fragments per kilobase of exon model per million mapped fragments. The values are presented as the means ± SDs. Asterisks indicate a significant difference determined via a t test (*P <0.05, **P <0.01,***P <0.001)