Fig. 1

miR528 enhances cold tolerance in banana. a-b Phenotype, lightness (L*) and chilling injury index of transient OE-miR528 and control banana peel slices under cold stress. c miR528 and MaPPO expression in the control and transient transgenic samples. U6 and Ribosomal protein S2 (RPS2) were used as internal references for miRNA and coding genes, respectively. d Changes in the PPO activity, MDA content and H₂O₂ level in control and transient transgenic samples. Error bars indicate the standard deviation (SD) of three or nine biological replicates (n = 3 or n = 9) and asterisks indicate significant differences between control and transient transgenic samples at each time point, as determined by two-tailed unpaired Student’s t-test (*, P < 0.05; **, P < 0.01, ns, not significant)