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Fig. 6 | Molecular Horticulture

Fig. 6

From: Transcription factor PbrERF114 is involved in the regulation of ethylene synthesis during pear fruit ripening

Fig. 6

PbrERF24 directly binds to PbrACS3 promoter. A The PbrACS3 promoter-driven LUC expression was enhanced through PbrERF24 activation. B The Y1H assay of PbrERF24 in the PbrACS3 promoter. C EMSA of PbrERF24 with the PbrACS3 promoter. + and − denote the presence and absence of recombinant PbrERF24-HIS, biotin-labeled probe, cold probe, and mutant cold probe, respectively. The cold probe concentrations were tenfold (10 ×), 50-fold (50 ×), and 200-fold (200 ×) those of the labeled probes. D Kinetic assay of PbrERF24 with the PbrACS3 promoter

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Molecular Horticulture

ISSN: 2730-9401

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