Fig. 5

PbrERF114 directly binds to the PbrERF24 promoter. A Predicted GCC-box elements were identified in the PbrERF24 promoter. B PbrERF114 induced LUC expression under the control of PbrERF24 promoter. Standard errors and analysis of variance were determined using Student’s t-test. Single and double asterisks indicate significant differences at P < 0.05 and 0.01, respectively. C The Y1H assay assessed the interaction between PbrERF114 and the predicted GCC-box elements in PbrERF24 promoter. (D) EMSA examined the binding of PbrERF114 to the PbrERF24 promoter. + and − denote the presence and absence of recombinant PbrERF114-HIS, biotin-labeled probe, cold probe, and mutant cold probe, respectively. The cold probe concentrations were tenfold (10 ×), 50-fold (50 ×), and 200-fold (200 ×) those of the labeled probes. (E) Kinetic assay of PbrERF114 with the PbrERF24 promoter