Fig. 3

The nucleus-localized PbrERF114 positively regulates ethylene biosynthesis and PbrACS3 expression. A Subcellular localization of PbrERF114. PbrERF114-GFP fusion proteins were localized within the nucleus, as confirmed through DAPI staining. An empty GFP vector served as the control. Scale bar = 20 μm. B Fruit flesh transiently overexpressing PbrERF114 (OE) and the corresponding empty vector control pSAK277 (CK). Scale bar = 1 cm. C Fruit flesh with transiently silenced PbrERF114 (TRV) and the corresponding empty vector control TRV 1 + TRV2 (CK). Scale bar = 1 cm. Six biological replicates were maintained for ethylene production. Three biological replicates are used for validation through qRT-PCR. Standard error and analysis of variance were determined using Student’s t-test. Single and double asterisks represent significant differences at P < 0.05 and < 0.001, respectively