Fig. 3

Susceptibility to cold stress of vegetative tissue in different tomato RILs. A, B Cold sensitive (71, 135, 150) and cold tolerant (47, 65, and 99) RILs were grown in perlite, and young plants (~ 30 days old; ~ 20–30 cm tall) were incubated at 1.5°C in the light for three days. Cold sensitive (5, 71, 90, 150) and cold tolerant (47, 49, 65, 99) RILs were grown in sand soil (C, D) or MS plates (~ 10 days old; ~ 8–10 cm tall) (E, F), and young plants were incubated in 1.5°C for 24 h followed with growth recovery at 25°C for 10 days. G Total chlorophyll content after 10 days of cold recovery of MS-grown plants. Bars with different letters indicate significant differences between sensitive and tolerant lines. One-way ANOVA p ≤ 0.05, as determined by Turkey-Kramer HSD