Fig. 3
From: 6-Methyl-5-hepten-2-one promotes programmed cell death during superficial scald development in pear
Identification of the PCD-related genes involved in scald development. a Expression profiles of 146 PCD-related genes and their correlation with scald incidence/index and MHO content. ‘Dangshansuli’ fruits were randomly divided into three treatments: H2O dipping (control), MHO fumigation, and DPA dipping. A total of 146 PCD-related genes are identified from pear genome based on transcriptome annotation (Table S2). Data, adapted from the transcriptome assay, present the mean values (Fragments Per Kilobase Million, FPKM) of three biological replicates. The color scale represents the normalized log2-transformed (FPKM + 1), where red indicates a high level, blue indicates a low level, and white indicates a medium level. Pearson correlations among attributes are visualized as a heatmap, with extremely strong negative correlations shown as green lines (correlation coefficients < -0.8) and positive correlations shown as red lines (correlation coefficients > 0.8). b qRT-PCR assay of the expression profiles of four PCD-related genes. The expression abundance of each gene in 0-d fruit was normalized to 1.0 for the qRT-PCR assay. The data are the mean values ± SD of three biological replicates. Vertical bars labeled with the same letter indicate no significant difference between samples at the same sampling time at P < 0.05. c The subcellular localization of PbrCNGC1 and PbrSOBIR1. OsMCA1-mcherry is used as the plasma membrane marker (Kurusu et al. 2012)