Fig. 4

MYB105 activates UFGT and promotes anthocyanin accumulation. A Yeast one-hybrid showed that MYB9/11/105 could all bind the promoter of ANR, but only MYB105 could bind the promoter of UFGT. B Schematic illustration of reporter and effectors used in the dual luciferase experiment. REN: reilla luciferase; LUC: firefly luciferase. 35S-driven REN was used as an internal control. 35S: the cauliflower mosaic virus 35S promoter; Ter: terminator. C MYB105 activates the promoter of UFGT. The vertical axis indicates the ratio of firefly luciferase enzyme activity to renilla luciferase enzyme activity. D Transient injection experiments in fruit showed that MYB105 promoted anthocyanin accumulation. E The anthocyanin content of the fruits indicated in (C). Bar in (D) was 1 cm. Data in (C) and (E) are mean ± SD from three replicates. Different letters indicate significant differences between different groups (one-way ANOVA, Tukey test, p < 0.05). *, p < 0.05, Student's t-test. FW: Fresh Weigh