Fig. 3

The anthocyanin pathway is activated in the anr mutant. A The number of differential expression genes (DEGs) between RG and anr mutant. up: up-regulated; down: down-regulated. B The heatmap of DEGs. rep1: repeat 1; rep2: repeat 2; rep3: repeat 3. C The enzyme genes related to anthocyanin synthesis were expressed at higher levels in the anr mutant than in RG. D The nine differentially expressed MYB transcription factors between RG and anr mutant. The vertical axis in (C) and (D) indicate the value of the FPKM in anr mutant relative to RG. FPKM: Fragments per Kilobase Million. The FPKM of genes in RG was normalized to 1. Data in (C) and (D) are mean ± SD, n = 3. **, p <0.01; ns, no significant; Student’s t-test. RG: Ruegen