Table 1 Applications of genome editing for agronomic traits improvement in potato. T, transcription activator-like effector nucleases (TALENs); C9, clustered regularly interspaced short palindromic repeats/CRISPR associated 9 (CRISPR/Cas9); C13, CRISPR/Cas13; RNP, protoplast transfection with ribonucleoproteins; CBE, cytosine base editor; PE, Prime Editor
From: Potato: from functional genomics to genetic improvement
Edit tool | Delivery approach | Genotype | Target gene | Objective | References |
|---|---|---|---|---|---|
T | A. tumefaciens | cv. Sassy | Sterol side chain reductase 2 (SSR2) | Reduction of SGA | Sawai et al. 2014 |
T | Protoplast | cv. Desiree | Acetolactate synthase (ALS) | Herbicide resistance | Nicolia et al. 2015 |
T | Protoplast | cv. Ranger Russet | Vacuolar invertase (VInv) | Reduction of cold-induced sweetening (CIS) | Clasen et al. 2016 |
T | A. tumefaciens | cv. Ranger Russet | Acetolactate synthase (StALS) | Herbicide resistance | Forsyth et al. 2016 |
T | A. tumefaciens | cv. Sayaka | Granule-bound starch synthase (GBSS) | Improving starch quality | Kusano et al. 2016 |
T | A. tumefaciens | cv. Shepody and cv. Russet Burbank | GBSS and vacuolar invertase (VInv) | Improving starch quality | Ma et al. 2017 |
T & C9 | A. tumefaciens | cv. Desiree and diploid self-incompatible breeding line, MSX914-10 | ALS | Herbicide resistance | Butler et al. 2016 |
C9 | A. tumefaciens | DM | Phytoene desaturase (PDS) and StAA2 gene (an Aux/IAA gene) | Proof of concept | |
C9 | A. tumefaciens | cv. Desiree and diploid self-incompatible breeding line, MSX914-10 | ALS | Proof of concept | Butler et al. 2015 |
C9 | A. tumefaciens | cv. Desiree | Transcription factor gene MYB44 | Functional genomics | Zhou et al. 2017 |
C9 | Protoplast | cv. Kuras | GBSS | Modification of starch composition. High amylopectin | Andersson et al. 2017 |
C9 | A. tumefaciens | S. tuberosum group Phureja S15-65 clone | S-locus RNase (S-RNase) | Elimination of reproductive self-incompatibility | Ye et al. 2018 |
C9 | A. tumefaciens | DRH-195 and DRH-310 | S-RNase | Elimination of reproductive self-incompatibility | Enciso-Rodriguez et al. 2019 |
C9 | A. rhizogenes | cv. Mayqueen | Steroid 16a-hydroxylase (St16DOX) | Nutritional quality. Reduction of toxic steroidal glycoalkaloids (SGAs) | Nakayasu et al. 2018 |
C9 | RNP | cv. Kuras | GBSS | Modification of starch composition. High amylopectin | Andersson et al. 2018 |
C9 | A. tumefaciens | cv. Sayaka | GBSS | Optimization of Cas9 expression with d-Mac3 translational enhancer | Kusano et al. 2018 |
C9 | Protoplast and A. tumefaciens | cv. Desiree | Starch branching enzymes (SBE1 and SBE2) | Modification of starch composition /nutritional quality. High amylose and longer amylopectin chains | Tuncel et al. 2019 |
C9 | Protoplast | cv. Desiree and cv. Wotan | GBSS | Modification of starch composition. High amylopectin | Johansen et al. 2019 |
C9 | RNP | cv. Desiree | Polyphenol oxidase 2 (StPPO2) | Post-harvest quality. Reduction of enzymatic browning | González et al. 2020 |
C9 | A. tumefaciens and RNP | cv. Desiree | StPPO2 | Reduction of enzymatic browning | González et al. 2021 |
C9 | A. tumefaciens | cv. Desiree and cv. King Edward | S-genes (StMLO1, StHDS,StTTM2, StDND1, StCHL1, StDMR6-1 and StDMR6-2) | Biotic stress tolerance. Resistance to Phytophthora infestans | Kieu et al. 2021 |
C9 | RNP | cv. Desiree | SBE1 and SBE2 | Modification of starch composition /nutritional quality. High amylose and longer amylopectin chains | Zhao et al. 2021 |
C9 | A. tumefaciens | cv. Yukon Gold TXYG79 | GBSSI | Decrease in amylose content | Toinga-Villafuerte et al. 2022 |
C9 | A. tumefaciens | cv. Sayaka | SBE3 | Low amylose | Takeuchi et al. 2021 |
C9 | A. tumefaciens | cv. Desiree | SS6 | N/A | Sevestre et al. 2020 |
C9 | Protoplast | cv. Wotan, Saturna | GWD1 | N/A | Carlsen et al. 2022 |
C9 | A. tumefaciens | cv. Desiree | PDS | Bleached (albino) leaves | Bánfalvi et al. 2020 |
C9 | A. tumefaciens | cv. DMF1 | PDS | Bleached whole plantlets | Butler et al. 2020 |
C9 | A. tumefaciens | cv. Atlantic | SSR2 | 66% of WT tuber | Zheng et al. 2021 |
C9 | Protoplast | cv. Desiree | eIF4E1 | Partial resistance to PVY | Lucioli et al. 2022 |
C9 | A. tumefaciens | cv. B665 and B663 | Sli | Self-incompatible plants | Eggers et al. 2021 |
C9 | A. tumefaciens | cv. Phureja S15–65 | IT1 | Loss of tuberization | Tang et al. 2022 |
C9 | A. tumefaciens | cv. Russet Burbank | CCOAOMT | Improved resistance to late blight | Hegde et al. 2021 |
C13 | A. tumefaciens | cv. Desiree | P3, CI, NIB, CP | Improved resistance to PVY | Zhan et al. 2019 |
CBE | A. tumefaciens | cv. Desiree | ALS | Herbicide resistance | Veillet et al. 2019b |
CBE | A. tumefaciens | cv. Desiree | GBSS and ALS | Construction of A3A-PBE cytosine base editor | Zong et al. 2018 |
C & CBE | Protoplast & A. tumefaciens | cv. Desiree and cv. Furia | GBSS | Modification of starch composition. High amylopectin | Veillet et al. 2019a |
C & CBE | A. tumefaciens | cv. Desiree | GBSS and Downy Mildew Resistant 6 (StDMR6-1) | Proof of concept | Veillet et al. 2020 |
PE2 | A. tumefaciens | cv. Desiree | ALS1 | Improved herbicide resistance | Perroud et al. 2022 |