Fig. 6

MBW specifically controls NtDFR expression, MYB12 alone activates NtFLS and NtLAR expression by dual-luciferase assay and the contents of flavonol, (pro)anthocyanin and anthocyanin in the oeNtMYB12 and Ntmyb12 tissue. A Scheme of effector and reporter; B The fluorescent imaging detected the protein expression of NtMYB12-GFP, NtWD40-1-mCherry and NtbHLH1-CFP in the cotransformed tobacco leaves under microscope. C-E luciferase fluorescent image and activity of cotransformed tobacco leaves with oeNtMYB12-GFP/NtWD40-2-mCherry /NtbHLH1-CFP and NtFLSpro:LUC, F–H and NtLARpro:LUC, (I)-(K) and NtDFRpro:LUC relative to empty vector, respectively; L the contents of flavonol, proanthocyanin, and anthocyanin in the oeNtMYB12 and Ntmyb12 narcissus callus tissue and in cotransformed narcissus leaves with oeNtMYB12-NtWD40-1-NtbHLH1 relative to mock (WT). The error bar presented standard deviants of three biological replicates (n = 9). Asterisk presents the statistical significance compared to empty vector (C-K) or WT (L) (* p < 0.05; ** p < 0.01; ***p < 0.001)