Fig. 3

The KEGG pathways of DEGs of Ntmyb12 or oeNtMYB12 relative to WT (IFCIā>āā=ā2, P valueā<ā0.01) were enriched in phenylpropanoid biosynthesis and flavonoid biosynthesis pathways. A The tissues used for RNA-seq; B Down-regulated enrichment pathways of DEGs from Ntmyb12 relative to WT by KEGG analysis; C Up-regulated enrichment pathway of DEGs from overexpressing NtMYB12 line relative to WT by KEGG analysis; D The heatmap of DEGs mapped in the flavonol and anthocyanin biosynthesis pathways; E The expression levels of NtFLS, NtLAR, NtF3H, NtDFR, and NtANS were indicated by RT-qPCR. The error bar presented standard deviants of three biological replicates (nā=ā9). Asterisk presents the statistical significance compared to WT (* pā<ā0.05; ** pā<ā0.01; ***pā<ā0.001)